The study of hepatotoxins in aqueous ecosystems of Western Greece

This project focuses on the evaluation of hepatotoxins Microcystin concentration in aqueous ecosystems of Western Greece. MC is known to be synthesized from cyanobacteria and it is highly toxic on liver cells. According to many researches, high and chronic MC consumption causes irreversible liver damage and ultimately could lead to the development of hepatocellular carcinoma.


PRESENTED ON: 13.07.2010

Undergraduate project

Supervised by Prof Evangelos Briasoulis

Run by Anastasia Touka

“The molecular screening of a four member family with stomach adenocarcinoma for heterozygote germ line mutations of the tumor suppressor gene CDH1”

The genetic basis for the great phenotypic variance of stomach cancer is thought to be the absence and/or inhibition of E-cadherin protein in the neoplastic tissue which is known to be coded by the CDH1 tumor suppressor gene. Two out of four individual members of a family who were diagnosed with stomach carcinoma were chosen for the purposes of this project.


PRESENTED ON: 12.10.2010

Undergraduate project

Supervised by Prof Evangelos Briasoulis

Run by Maria Igglezou

“The molecular screening of a four member family with stomach adenocarcinoma for heterozygote germ line mutations of the tumor suppressor gene CDH1”

The genetic basis for the great phenotypic variance of stomach cancer is thought to be the absence and/or inhibition of E-cadherin protein in the neoplastic tissue which is known to be coded by the CDH1 tumor suppressor gene. Two out of four individual members of a family who were diagnosed with stomach carcinoma were chosen for the purposes of this project.


PRESENTED ON: 13.07.2011

Undergraduate section

"Microcystin-RR cytotoxicity on cancer cells. A hypothesis evaluated by xCELLigence system technology"

Microcystins are Cyanobacterial Cyclopeptides found in fresh water. The main endocellular events caused by MC exposure have been studied by many scientists. The main purpose of this project was to evaluate and to study in real time the probable cytotoxic effects of MC-RR on cancer cells using the xCELLigence system.


PRESENTED ON: 09.11.2011

Undergraduate project

Supervised by Prof Evangelos Briasoulis

Run by Evangelia Karastergiou

"Microcystin-LR cytotoxicity on cancer cells. A hypothesis evaluated by xCELLigence system technology"

Microcystins are Cyanobacterial Cyclopeptides found in fresh water. The main endocellular events caused by MC exposure have been studied by many scientists. The main purpose of this project was to evaluate and to study in real time the probable cytotoxic effects of MC-LR on cancer cells using the xCELLigence system.


PRESENTED ON: 09.11.2011

Undergraduate project

Supervised by Prof Evangelos Briasoulis

Run by Eleftheria Kazepidou

Undergraduate project

Supervised by Prof Evangelos Briasoulis

Run by Lambrini Tsoutsi

The use of High Resolution Melting Analysis (HRMA) technology in the evaluation of SLCO1B3 gene mutation.

The over expression of Organic Anion Transporter Polypeptide 1B3 (protein symbol OATP1B3, gene symbol SLCO1B3) has been shown in several types of cancers, such as breast cancer, lung cancer,  pancreatic cancer and colon cancer. The current project focused in the evaluation of SLCO1B3 mutations in healthy individuals and in cancer patients using HARMA. High Resolution Melting Analysis is a method which is used to detect mutations, polymorphisms, small imports or subtractions of bases and epigenetic differences in genomic DNA in vitro.


PRESENTED ON: 13.07.2011

Undergraduate project

Supervised by Prof Evangelos Briasoulis

Run by Petros Poulakis

graphic design by Julie Yiokka

Undergraduate project

Supervised by Dr Ioannis Sainis

Run by Eleona Liti

“Assessing the similarity of cyanobacterial communities in alpine lakes of Epirus region (NW Greece) by using molecular techniques”

Alpine lakes represent ecosystems of great research interest, due to their isolation. The fact that their origin dates back to only a few thousand years ago, at the end of the last glacial period, suggesting that their biodiversity assemblages derived from a process of recent colonization from neighbouring geological older habitats. The main goal of this study is to determine the resemblance among the cyanobacterial communities in alpine lakes in the Epirus region (Lake Gistova, Verliga and the lake of Smolikas) and their resemblance with those of Lake Ohrid, the most ancient lake in the Balkans, by using molecular techniques.

PRESENTED ON: 17.12.2013

“Molecular study of the cyanobacterial diversity in lakes Amvrakia and Trichonis (Greece)”

Cyanobacteria play an important role in the recycling of nutrients in ecosystems, but their blooms may be hazardous for humans and animals. The aim of this study is to characterize and compare the cyanobacterial species composition in the two largest lakes in the province of Aetoloakarnania, Amvrakia and Trichonis by using molecular techniques and bioinformatics.



PRESENTED ON: 08.07.2014

Undergraduate project

Supervised by Dr Ioannis Sainis

Run by Dimitra Koumasi

“Exploring Biodiversity in the Microbial Community (Bacteria-Archaea) of the Mediterranean Phyllosphere and its Relationship with Airborne Bacteria”

The structure and diversity of phyllosphere microbial communities (Bacteria and Archaea) attracts scientific interest. In this project, we selected nine dominant perennial species, namely Arbutus unedo, Cistus incanus, Lavandula stoechas, Myrtus communis, Phillyrea latifolia, Pistacia lentiscus, Quercus coccifera (woody), Calamintha nepeta, and Melissa officinalis (herbaceous) and we were interested to study microbial communities in phyllosphere and air under the harsh conditions of the Mediterranean summer. We also examined the extent to which airborne bacteria resemble to the epiphytic ones

PRESENTED ON:

Undergraduate project

Supervised by Dr Ioannis Sainis and

                          Dr Nikolaos Monokrousos

Run by Despoina Voggoli

“Perioperative kinetics of circulating microRNAs in patients undergoing mastectomy for breast cancer”

Cancer is, nowadays, one of the leading causes of death, with breast cancer to be the most common among women, worldwide. MicroRNAs are endogenous ssRNA molecules, sizing from 19 to 25 nucleotides and they are post-transcriptional regulators of gene expression, in normal and pathological conditions, such as in cancer. Because of this, they are under investigation for becoming possible biomarkers of the disease. The aim of the present study is to study the perioperative levels of two microRNAs, whose role in cancer has been proven, miR-155 and miR-21, in patients with breast cancer undergoing mastectomy, using the Real Time PCR. 


PRESENTED ON: 08.07.2013

Undergraduate project

Supervised by Prof Evangelos Briasoulis

Run by Marina Drolia

“Study of apoptosis regulatory microRNAs in patients with acute myeloid leukemia with PCR Arrays method”

Apoptosis plays a critical role in normal biological processes requiring cell removal including differentiation, development, and homeostasis. Stress responses (such as heat shock, ischemia, unfolded proteins, and viral infection) cause badly damaged cells to undergo apoptosis. An acquired defect in apoptosis activation often leads to uncontrolled cell growth, oncogenesis, and cancer. Βesides these post-translational events, miRNAs provide another mechanism of regulating apoptosis by attenuating gene expression. We are going to study 84 microRNAs, with the miSCRIPT PCR arrays plate. This array represents miRNAs experimentally verified to regulate many pro- and anti-apoptotic genes and to stimulate or attenuate apoptosis upon their overexpression or inhibiton. Monitoring their expression helps determine the mechanisms behind programmed cell death in any model system and the propensity of a cell type to undergo apoptosis normally. This array provides researchers with a convenient way to quickly analyze the miRNAs most relevant to apoptosis.


PRESENTED ON:

Undergraduate project

Supervised by Prof Evangelos Briasoulis

Run by Andreas Katsenos

“The study of plasma circulating microRNAs in patients with colorectal cancer (CRC) ”

MicroRNAs were discovered 20 years ago but continue to be an important matter of research. They are related to the most of the biological processes such as apoptosis, organogenesis.  However, it has been found to play an important role in cancer. Colorectal cancer (CRC) is the second most common cancer in females and the third in males with 1.2 million annual new cases worldwide. The expression of specific miRNAs in plasma has been reported in different types of CRC. Among all microRNAs studied, it seems that miR-92a is significantly upregulated in CRC patients in plasma-based studies. We are going to study the expression of specific microRNAs in plasma of patient with CRC before the surgery and after.


PRESENTED ON:

Undergraduate project

Supervised by Prof Evangelos Briasoulis

Run by Nantia Anastasiou

Perioperative kinetics of plasma circulating microRNAs in patients with gastric cancer.

MiRNAs are worth pursuing in research and treatment of gastric cancer because of their diagnostic and prognostic significance. Gastric cancer ranks second among the most common cancers.  The purpose of this research is the isolation and quantification of circulating miRNAs) in a pre-operative and post-operative stage within the plasma of patients diagnosed with gastric cancer. The relative quantitative expression of these is achieved with the Real-Time-PCR method. Up-regulation or down-regulation of miRNAs in the plasma may be helping us to determine  their prognostic value.   



PRESENTED ON:

Undergraduate project

Supervised by Prof Evangelos Briasoulis

Run by Panagiota Erotocritou

“Circulating microRNAs in blood from patients with colorectal cancer”

MicroRNAs are small non-coding RNAs and regulate the expression of approximately 30% of protein-coding genes. A single microRNA can target hundreds of mRNAs.  Specifically, miRNAs are bind with a complex of proteins named RISC which transfer them to the 3’ UTR region of mRNAs. Several cellular processes  like cellular development, differentiation, proliferation and cell death are regulated by microRNAs. For this reason, dysregulated microRNAs could contribute to the initiation and progression of a variety of malignancies, including solid tumors. Furthermore, miRNAs have been shown as potential signatures for the classification, diagnosis, and progression of cancer. Colorectal carcinoma (CRC) is one of the leading causes of cancer-related death worldwide. They have investigated plenty of microRNAs on body fluids like blood, plasma. Some of these microRNAs are miR-134, miR-221, miR-25 and miR-92a.  There is also some microRNAs that are related with angiogenesis like miR-107, miR-145and miR-27a. The intention is to investigate the expression levels of circulating microRNAs that are related with colorectal cancer, and compare their expression levels between patients ( pre-operative and post-operative samples)  and healthy.


PRESENTED ON:

Undergraduate project

Supervised by Prof Evangelos Briasoulis

Run by Theodora Lontra

Selection of CD34+ cells, and investigation of quality of extracted mRNA, miRNA, DNA and protein of bone marrow samples from patients with acute leukemia stored in liquid nitrogen in Cancer Biobank Center of the University of Ioannina.


Isolation of mononuclear cells, and optimal processing and storage of peripheral blood or bone marrow of patients with Acute Leukemia is crucial to conducting translational research studies in this disease. Isolation of mononuclear cells was performed using ficoll gradient centrifugation followed by gradual stratification of blood or marrow components. In order to select CD34+ cells we used magnetic beads protocol. That was followed by isolation DNA and RNA  of the labeled and eluted cells with consequent control of the quality and quantity of these biomolecules. We found that for at least two years post storage in liquid nitrogen we could obtain leukemia-specific biomolecules of excellent quality to be used in translational research studies when combined with proper clinical data.

Undergraduate project

Supervised by Prof Evangelos Briasoulis

Run by Filio Patsalosavi

Circulating levels of miR-155-3p and miR-155-5p in colorectal cancer patients and healthy individuals



Among the many microRNAs, which are related to the colorectal cancer miR-155 is the most established. We investigated the circulating levels of miR-155-3p and miR-155-5p in patients (preoperatively and postoperatively) and compared with a control group of healthy individuals.

We found that circulating levels of miR-155-3p but not of miR-155-5p are higher in patients with colorectal cancer compared to healthy individuals

Undergraduate project

Supervised by Prof Evangelos Briasoulis

Run by Iliana Drouga

Differentially expression of Let-7a in men and women


Let-7a, one of the let-7 miRNA members, has been found differentially expressed in male and female gonadal tissues in many animal studies. More specifically, gonadal let-7 was found to be upregulated in male animals compared to female and testosterone tended to increase the expression of this microRNA. We investigated the circulating levels of let-7 in young adult individuals and found the circulated levels of let-7 are significantly higher in men compared to women.

Undergraduate project

Supervised by Prof Evangelos Briasoulis

Run by Eleftheria Siola

Circulating levels of the miR-135 in gastric cancer patients and healthy individuals

The mirRNA are expressed with a tissue specific way and each one of them has different target genes, resulting in the increased complexity of the regulatory network. The aim of the study was to investigate circulating levels of the miR-135 as a potential diagnostic biomarker in gastric cancer. We found that pre-surgical blood miRNA-135-5p levels in gastric cancer patients are higher compared to post-surgical levels and significantly higher compared to those of healthy controls.

Undergraduate project

Supervised by Prof Evangelos Briasoulis

Run by Elena Neofytou

Circulating levels of miR-21-3p and miR-215p in plasma of patients with colorectal cancer and healthy individuals

The main objective of this study was to investigate pre- and post-surgical plasma levels of miR-21-3p and miR-215p that have been associated with colorectal cancer, and compare with healthy individuals. We failed to demonstrate significant expression of plasma levels of studied miRNAs in both study groups.

Undergraduate project

Supervised by Prof Evangelos Briasoulis

Run by A. Giulten